Figure 1. Cellular localization of ANXA1-wild-type-enhanced GFP and ANXA1-S²⁷/A-EGFP mutant in TtT/GF cells. A) Unstimulated cells transfected with the empty plasmid EGFP, ANXA1 WT-enhanced GFP, or ANXA1 S²⁷/A-enhanced GFP. Nuclei were stained with topro 3. Merged images represent EGFP-topro3. Cells were fixed and moviol mounted. B) Cells stimulated with LPS (100 ng/ml, 5 min). All images were collected at similar exposure time. All images file were processed for presentation using Image Pro plus software (Media Cybernetics) and imported directly in PowerPoint in publication format. Arrows indicated area of ANXA1 localization after LPS treatment.