Figure 1. Changes in GM3 levels during glutamate-induced cell death. A) Ganglioside extracts from HT22 cells treated with 5 mM glutamate for the indicated times were applied to HPTLC plates and visualized by resorcinol staining. B) Flow cytometric analysis of GM3 levels on HT22 cell plasma membrane was performed. HT22 cells were incubated with or without 5 µM FCCP or 20 µM cobalt chloride (Cobalt) in the presence of 5 mM glutamate for 8 h and incubated with anti-GM3 (GMR6), followed by the addition of FITC-conjugated antimouse immunoglobulin. Data represent six experiments (means±SD). *P < 0.005, compared with groups of respective control.