Figure 2. CaMK inhibition increases H₂O₂-induced apoptosis. S-phase synchronized HUVEC were treated with CaMK inhibitor KN93 (10 µmol/l) or solvent alone followed by incubation with or without 400 µmol/l H₂O₂ for additional 24 h. Apoptosis was measured by calculating percentage of cells displaying subdiploid DNA content (left, n=11) or the apoptotic DNA fragmentation (right, n=4). In cells incubated with KN93 and H₂O₂, apoptosis significantly increased over both cells treated with H₂O₂ alone or KN93 alone.