Figure 1. Radioactive lipids from mock Neu3 and cells fed with radioactive GM3. Mock and Neu3-transfected cells (lane 1) were treated with ammonium chloride 10 mM (lane 2) or chloroquine 50 µM (lane 3) or kept at 4°C (lane 4). Then the cells were fed with [³H]GM3. Lipids from mock and Neu3-transfected cells were extracted and separated by HPTLC.