Figure 2. Effects of free radical scavengers on mechanical strain-stimulated cardiomyogenesis (A) and vasculogenesis/angiogenesis (B). 4-day-old embryoid bodies were preincubated for 2 h prior to mechanical strain (10%) application with either NPMG (100 µM) or vitamin E (100 µM). Free radical scavengers were present in the cell culture medium until inspection. Note that in the presence of free radical scavengers, mechanical strain-stimulated cardiomyogenesis as well as vasculogenesis/angiogenesis were significantly inhibited, pointing toward an involvement of ROS. *P < 0.05, significantly different as indicated.