Figure 2. NO down-regulates PGC-1 mRNA through a PKG-dependent mechanism. BAEC were treated as follows. A) DETA-NO (62 µM) ± the soluble guanylate cyclase inhibitor ODQ (1 µM), B) DETA-NO (62 µM) ± the protein kinase G inhibitor KT5823 (1 µM). A, B) Upper panels show PGC-1 mRNA expression at 6, 12, and 24 h of treatment. Lower panels show mRNA expression of PGC-1 target genes after 12 h of treatment. Expression in untreated samples has been assigned the value of 1. *P < 0.05, #P < 0.001.