Figure 1. Quantification of adventitial cell migration Localization of ß-gal transgene expression was determined by X-gal staining and the proportion of ß-gal positive blue nuclei in the neointimal compartment at 3, 7, and 14 days after balloon injury was quantified using chromagen separation PC software in micrographs of AdGFP and Adß-gal (open bars) or AdPDGFXR and Adß-gal (solid bars) transfected arterial sections, captured using a microscope fitted with a CCD camera. Data are mean ± SE; n = 6–12 animals per treatment time point, *P < 0.05.